Coding

Part:BBa_K866000:Design

Designed by: Michael Boelker   Group: iGEM12_Marburg_SYNMIKRO   (2012-09-25)

GFP fusion brick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641

Design Notes

The two nucleotides TG of the start codon from the CFP brick were deleted to create this fusion brick via site-directed mutagenesis with the following primer:

Primer name Primer Length G/C content Tm
MS 16 5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3' 43 bp 62.7% 90.3 °C
MS 17 5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3' 43 bp 51.1% 80.5 °C

Source

The CFP fusion brick was derived from the CFP BioBrick, BBa_E0020.

References